The committee has also included an additional counter-cyclical buffer of up to 2.5% of risk-weighted assets, which regulators can impose when they think credit is flowing freely.
We included an additional high-salt extraction step using buffer C (10 mM HEPES, pH 7.9, 10 mM KCl, 300 mM NaCl, 1.5 mM MgCl2, 25% glycerol, 0.1% Triton X-100, 1 mM DTT, 10 µg/ml protease inhibitor cocktail, and 0.4 mM PMSF).
However, the second protocol included an additional step of washing with saline buffer just after sample rehydration.
Earth's atmosphere provides an additional buffer against all but the most energetic of particles.
CSF provides a special chemical environment for nervous tissue, as well as an additional buffer against physical damage.
They butt heads with planning officials who see a line of curbside trees as providing an additional buffer between cars and pedestrians and creating a space where plowed snow can pile up without impeding foot traffic.
The facility will hold an "additional buffer" of stock to ensure essential health and care would continue.
All other nodes require an additional buffer for one maximum sized packet.
It might not be obvious, but nearly every element boasts fire-resistant properties, creating an additional buffer.
The recovered IDEPT agent was subjected to an additional buffer exchange step using an additional Zeba Spin Desalting Column.
Include an additional wash with 750μl of 35% guanidine hydrochloride prior to the Buffer PE wash step.
