"The result is a network composed of two blocks: one includes the susceptible as disconnected singletons, while the other includes the infected individuals which are interconnected," says the Santa Fe group.
To construct the model for migratory birds, we suppose that the prey population, including the susceptible population, S, and the infected population, I, migrate into the system.
For our mapping population, however, other than the parental lines we also included the susceptible check Swarna-Sub1 and the tolerant check IRRI154 which can also be used as negative and positive checks, respectively, when we investigate the performance of the selected individuals in the mapping population.
The model includes the susceptible strain and a strain that is resistant to antibiotic A. Antibiotics are administered for a total of 9 days to optimize the duration of therapy, as shown in Figure 1.
[GenBank: AX196295.1] includes the susceptible allele of soybean rhg1 gene on molecular linkage group G. [GenBank: AX197297] includes the susceptible allele of soybean Rhg4 gene on molecular linkage group A2.
Thus, a total of 56 naturally occurring Pm3 sequences are now known, including the susceptible ancestral sequence Pm3CS (found in both tetraploid and hexaploid wheat species).
Primers were designed to amplify the full length of the four candidate genes in ten resistant inbred lines (including the resistant parental line) and ten susceptible inbred lines (including the susceptible parental line) (Table 3).
We noted a basal expression pattern of both genes in the young root tip, including the susceptible zone and did not observe a discernible change in expression pattern of neither MtCCD7 nor MtCCD8 following LCO treatment (Additional file 4).
To further identify which one was the most likely candidate gene for CFW resistance, primers were designed to obtain both full length DNA and cDNA of the two genes from ten resistant cabbage inbred lines including the resistant parent (R_bulk) and ten susceptible lines including the susceptible parent (S_bulk) in order to conduct sequence alignment and mutation loci analysis (Table 4).
To identify which one was the candidate gene, primers were designed and used to amplify the genomic DNA and cDNA of ten resistant cabbage inbred-lines (R_bulk) including the resistant parent P1 and ten susceptible lines (S_bulk) including the susceptible parent P2.
