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The Arabidopsis and rice proteome data include alternatively splicing variants, while all the other proteomes do not.
The few published genome-wide expression studies on non-model species primarily focus on differential expression at the gene level and de novo assemblies do not include alternatively spliced transcripts e.g. [ 29].
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All CDSs, including alternatively spliced forms, were considered for estimation of the average and median CDS lengths.
HCDiff callings presume at least three reads with the variant with both forward and reverse reads included; alternatively the quality scores at the variable positions must be over 20 (or over 30 if a homopolymer of five or more bases is involved).
Gene annotation of these sequences predicted 87 putative genes, including alternatively splicing transcript forms.
Here, we show that DNA methylation is significantly enriched in included alternatively spliced exons (ASEs) as compared with excluded ASEs.
Normalization of the cDNA library led to the enrichment of rare transcripts in U. vulgaris, including alternatively spliced mRNAs.
It was confirmed that the 318 bp product includes alternatively spliced exon 6, whereas the 282 bp product excludes it.
In all models (OLS and Quantile Regression) renal function was included alternatively as a continuous (eGFR) or categorical variable.
Trinity assembler generates high numbers of putative transcripts, including alternatively spliced isoforms and transcripts from recently duplicated genes which lead to the generation of similar transcripts [ 48].
These included alternatively spliced genes such as longitudinals lacking (lola) (Neumüller et al., 2011), but also many genes for which tissue-specific alternative splicing has not been described.
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