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The diagnosis was not, however, confirmed because an inappropriate specimen (a CSF specimen taken on admission) was submitted.
Reliable tests for pathogens like Campylobacter may be compromised by inappropriate specimen collection or preparation procedures (24 ) or by antimicrobial therapy before stool collection.
Of the 25,083 specimens from Alberta, 1,377 (5.5%) were also excluded because pertussis testing was not performed due to reasons that include inappropriate specimen types or transport media, incomplete patient or submitter information, and leakage during specimen transport.
Similar(57)
However, PCR inhibitors are frequently encountered in severely ill patients or in inappropriate specimens.
In the six months following implementation there were significant decreases in the proportion of inappropriate urine specimens sent for culture, episodes of treatment of asymptomatic bacteriuria, and total antimicrobial days.
Considering the preanalytical mistakes, the most common faults (Table 4) depended on inaccurate procedures for sample collection, including inappropriate quality of specimen (47.04 %), inappropriate quantity of specimen (11.55 %), and utilization of an inappropriate container (0.57 %).
Inappropriate sampling or specimen processing or suboptimal sensitivity of the PCR assay used most probably accounted for the negative results.
In addition to the infected cases, 3 further acetabula were excluded from histological assessment, as the sectioning of the specimen was inappropriate.
Thus, specimens with inappropriate rubricytosis could possibly be missed with our approach.
Therefore, we feel that the results obtained by the authors might be flawed by the inappropriate processing of the specimens.
This result may reflect inappropriate long term stored specimens (as a retrospective study), and/or the presence of mutations in the target regions of probes.
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