Toxicity is a greater problem when considering a two-hybrid system (or other expression library) of prokaryotic origin, since inappropriate expression of an E. coli gene is frequently a problem, particularly for genes encoding components of multisubunit complexes and transmembrane proteins.
Even the city's origins seem inappropriate for a national capital: its earliest historical role was as the site of a small Moorish fortress on a rocky outcrop part of the northern defenses of what was then the far more important city of Toledo, located about 43 miles (70 km) south-southwest.
Geminin acts to prevent a second, inappropriate firing of replication origins by competitively binding to Cdt1 and thereby blocking recruitment of the Mcm2 7 helicase to replication origins [ 39, 48].
Activated CHK1 then plays pivotal roles in coordinating cellular responses to replication stress, slowing S-phase progression by suppressing inappropriate firing of replication origins, maintaining fork integrity, facilitating resolution of stalled forks and triggering G2/M arrest.
The term Rhodesia derived from the surname of Cecil Rhodes, the primary instigator of British colonisation of the territory during the late 19th century was perceived as inappropriate because of its colonial origin and connotations.
Recognition that such weakness may be of spinal origin may prevent inappropriate treatment and diagnostic testing.
Proponents of the initiative also say government inquiries about race, ethnicity, and national origin are as intrusive and inappropriate as questions about religion and sexual orientation.
And through contact status analysis, inappropriate gap design was regarded as the origin of the too large stress, which was the primary cause of these anomalies during firing test.
In more than half the cases, the inappropriate use of antidepressants was at the origin of the non concordance of treatment with respect to guidelines.
The use of municipalities as origins and destinations may be inappropriate for performing a good spatial analysis, as there are few resulting relationships.
