The p53, p38 and p21 antibodies used in subsequent assays were tested by immunocytochemistry, but proved inadequate for quantification due both to a diffuse distribution throughout the cell body and cell-to-cell variation in intensities that appears to result from induction of stress pathways in some cells during transfection (data not shown).
In that case, one could still acquire 'enough counts', but these are then of little value since image quality will be poor, and quantification will be inadequate.
In our preliminary tests, our entry-level mass spectrometer showed an inadequate sensitivity for quantification of serum 25(OH)D2 and 25(OH)D3 using the kit in nonderivatized mode (data not shown).
However, their contribution to climate change mitigation through carbon storage is not obvious because of limited information regarding their extent, and inadequate methods for biomass quantification.
The main weak point is the inadequate phenotype selected for quantification and consequent overstatements in the interpretation of the obtained data.
In the context of medical decision-making, many aspects of maternity care are characterised by inadequate evidence, in particular, the quantification of the risk of adverse outcomes associated with births in different settings.
X-ray diffraction and Rietveld amorphous bulk quantification proved to be an inadequate methodology to quantify the effects of mechanical activation.
These include (1) their low and unstable yields [16, 86]; (2) inadequate policy support [14]; (3) lack of proper quantification (and recognition) of long-term benefits of legumes within cropping systems [82].
Identification of elevated plantar pressure without equipment for quantification of plantar pressure is inadequate.
This seems quite unlikely, and contrary to the plant symptoms, indicating that the absolute quantification with no normalisation is inadequate to quantify viruses in plants.
While the limited use of absolute quantification has to date provided an inadequate context for universally comparing gene expression levels, EF1α does provide some perspective as to the magnitude of candidate gene expression.
