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Remarkably, despite inadequate normalization of sigma es), cardiac function as measured by serial echocardiography showed little deterioration in either of the pressure-overloaded genetic models with blunted hypertrophy.
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First, the circuit blood flow employed by Livigni and coworkers is only 5% of the cardiac output, which was inadequate to achieve normalization of arterial carbon dioxide pressure (PaCO2).
It is notable that many of the genes downregulated in the pregnant C. ocellatus uterus are genes involved with basic cellular housekeeping processes (e.g., ribosomal subunit proteins, desmin, myosin; table 3), which could indicate that the TMM normalization was inadequate and that these genes were undersampled.
Remarkably, we found that the majority of genes in the genome appeared to change expression and consequently our usual data normalization methods were inadequate.
These results support the concept that inadequate microcirculatory perfusion may persist despite normalization of systemic hemodynamics and that this contributes to poor outcome.
This inadequate response persisted during the 96 h of this study despite normalization of the acid base status.
Conclusion Inadequate kidney response to metabolic acidosis was frequent in septic patients and persists despite normalization of the acid base status.
Contrary to initial beliefs that miRNA-seq data will not require sophisticated normalization [ 45], we and others have observed that simply adjusting miRNA counts to the sequencing depth is inadequate [ 17, 18].
Inadequate, inadequate, inadequate.
Revitalization vs. Normalization.
B. Group data following normalization.
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