Control experiments demonstrate that the activity of ToyJ requires intact protein, as boiled ToyJ is inactive (Supporting Figure S6, Supporting Information).
We speculate that euchromatic F insertions are transcriptionally inactive (supported by very low RNA-seq RPKM levels) and devoid of H3K9me3 marks (hence no H3K9me3 spreading) and that the high H3K9me3 levels for the average F-element stem from the abundant heterochromatic insertions.
Silver oxide, on an inactive, refractory support, catalyzes oxidation of ethylene to ethylene oxide.
Active SMS deposits host endemic hydrothermal vent communities, whilst inactive deposits support communities of deep water corals and other suspension feeders.
The extensive interactions of this rare sequence explain why common DS−GAG sequences (with unsulfated IdoA p) are inactive and support the idea that the hexasaccharide D6−HCII interaction is specific.
Also, the activity of citrate synthase tended to be higher (p = 0.053, in muscle from active than inactive men), supporting an increased capacity for flux through the TCA cycle in muscle tissue of active men (Fig. 2C).
However, the finding, that the protein level of the upstream regulator, GSK3β, was significantly higher in the group of physically active than in the inactive men, supports a role of insulin- and/or Wnt-mediated GSK3 signaling in harnessing of the promoter potential of metabolic genes (Fig. 3A,B).
10.7554/eLife.03751.014 Figure 2. Catalytically inactive AKT supports the survival of AKT-dependent human cancer cells.
In this experiment, radicicol demonstrated an EC50 value comparable to 17-AAG, while as expected KU298 was inactive, further supporting the specificity of this assay for Hsp90 inhibition.
Our data showing that speed of movement on an ICAM-1 substrate is the same for both Zap70 catalytically active and inactive CTL supports the idea that integrin activation is independent of Zap70 catalytic activity.
