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Subsequently, the virus was inactivated using BEI as described before [ 25], and inactivated virus was stored at −70°C.
Inactivated VEEV particles were used to ensure that the antibodies selected on active VEEV particles bound inactivated virus, too.
Barouch's team tested the body fluids of monkeys that received the inactivated virus.
"It is an inactivated virus but not yet a vaccine," the scientist said.
"There's a suspicion that it might be less immunogenic than whole inactivated virus," Barouch concedes.
Before long, the first batch of inactivated virus was shipped from the Walter Reed Institute to Barouch's lab.
The formaldehyde is then removed, and the inactivated virus is packaged in rubber-topped glass vials, ready for inoculation.
Similar(4)
This effect was markedly reduced when the same MOI of UV-inactivated virus was used, and almost completely negated by heat inactivated virus.
Aliquots of infectious and UV-inactivated virus were stored at −80°C until use.
Control macaques inoculated with heat-inactivated virus were consistently negative for SIV.
Therefore, we next investigated the effects of UV-inactivated virus on the HIF-1 α target genes.
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