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mrc1 AQ is a mutant allele in which SQ/TQ residues have been substituted with AQ, resulting in its inability to mediate phosphorylation and activation of Rad53 [18].
We found that despite MPA inability to mediate GATA3 transcriptional repression in T47D-Y-C587A cells total GATA3 protein levels were still downregulated by MPA in this cell line.
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This led us to wonder if the inability of the GLS to mediate AD localization was due to its inability to bind the transport machinery tightly.
We next wondered whether the inability of the GLS to mediate AD localization was related to the fact that KGFP transcripts, with or without the GLS and/or TLS, are translated.
Alternatively, differences in the efficiency of RNAi within distinct tissues may account for the inability of certain MPLS genes to mediate suppression for some phenotypes.
Since the MEF5-/ cells express endogenous STAT1 and STAT3, the specificity of this reporter was demonstrated by the inability of the His-vector to mediate luciferase activity.
Therefore it remains unclear whether the absence of Cx43 knockdown in our tests can be attributed to the inability of siRNA delivered by our formulation protocol to mediate functional effect in the retina.
The ability of cytochalasin D to inhibit cytosolic luciferase accumulation in KG-1 cells, as well as the inability of the non-macropinocytotic CEM cell line to mediate the process, are consistent with this idea.
"This is not a bad thing, but there has been an inability to find a balance between arts, culture, commerce and the residents, and a lack of any entities to mediate this dialogue.
The imam tried to mediate.
He also offered to mediate.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com