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These structural clues and in-silico tests suggest possible avenues for improving the binding affinity of ABT263 through further in-vitro and in-vivo tests.
The four in-silico tests—related Supplementary Videos 6 9 are provided—, which could be considered as limiting cases of real scenarios where mixed boundary conditions are expected (compliant clamps), are all in agreement with the buckling-hinge model prediction, as demonstrated in the Supplementary Fig. 4, confirming the generality of the proposed approach.
To evaluate the performance of our hybrid model, several in-silico tests were performed including comparison with a data-driven (regression) model (Additional File 3, S2a/b) and assessment of model sensitivity to i) optimisation parameters, ii) experimental design, iii) data deterioration, iii) generic topology.
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The hypotheses formulated during in-silico testing suggest new avenues to explore experimentally.
In silico tests of the primers revealed at least 38% higher coverage for Archaea compared to other commonly used primers.
The discriminatory efficiency of all SMIRs was demonstrated with different "in silico" tests, using available databases, showing that all SMIRs have enough informative resolution for species identification, even for closely related species from the same genus.
First, in silico tests were performed which demonstrated that all designed primers 100% matched with database sequences of their respective groups, whereas lower matches with other non-target bacterial groups were found.
The in silico tests, performed on 4 different scenarios using a simulator equipped with 100 patients, show that the performances of MPC1 are very promising, supporting its current use in an in vivo multicenter trial on 47 patients that is being carried out within the European Research Project AP@home.
For these reasons the in silico tests were not exhaustive and only focused on trnH-psbA and rbcL.
In silico tests for recombination and in vitro mating experiments revealed a history of sexual reproduction for H. jecorina and confirmed clonality for T. parareesei nom.
The in silico tests performed in Sects.
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