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For in-house validation of new CRL-validated PCR tests, we therefore propose to use an initial set of in-house validation data using levels in a restricted dynamic range, matrices of comparable complexity and experiments of comparable duration to determine overall values for the vd and vi that can then be used for additional validation studies.
The HUMAN GPCR-DB, in addition to providing this set of information, provides in-house validation and assay-information for non-validated nsSNPs.
In compliance with the Commission Decision 2002/657/EC (CD 2002/657/EC), the method was validated applying a matrix-comprehensive in-house validation approach based on a fractional factorial design.
The matrix-comprehensive in-house validation concept is based on complex mathematic-statistical calculations.
Uncertainty assessment of measurement was estimated on the basis of an in-house validation according to EURACHEM/CITAC guide.
Another relatively quick and easy way of uncertainty estimation is the in-house validation that includes the determination of the method performance parameters (Baralkiewicz et al. [2013]).
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Requirements for in-house validations have also been discussed by Žel et al. [ 19].
A bias between −43% and +37% was found for the event-specific methods in these in-house validations (Table 6).
In the EU ring trials, either extracted DNA or flour samples of reference material were distributed and therefore only four of the in-house validations could be compared to ring trial results (marked in yellow in Table 10).
In this work, an in house validation of a difference spectrophotometric method for HMF analysis in corn syrups was developed using sophisticated statistical tools by the first time.
Performance characteristics were determined in a preliminary in house validation.
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