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The PSSM profile, HMM and COMPASS profile were searched against our in-house template sequence database, template HMM database and template COMPASS profile database to identify homologous templates from the output generated by PSI-BLAST, hhsearch and COMPASS, respectively.
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These issues highlighted a clear priority for shared care: setting up simple systems for proactive reviews should be favoured over the development of in-house templates and databases.
We normalized PET images using an in-house pediatric template (44 children with negative MRI, including 28 with focal epilepsy and 16 with degenerative-like neuro-Langerhans histiocytosis, mean age 10 ± 3 years) [36].
Consequently, each laboratory would have to develop its own in-house brain template.
The ADD images were then spatially normalized to an in-house RAC template in standard stereotaxic (MNI) space using statistical parametric mapping (SPM2) software (Wellcome Functional Imaging Laboratory, London).
It was enzymatically generated using a single-stranded DNA library synthesized in-house as template for a one-cycle fill-in reaction with Vent exo− DNA polymerase (New England Biolabs) and the T7 RNA polymerase promotor-containing forward primer 5′-TCTAATACGACT-CACTATAGGAGCTTAGACAACAGCAG-3′, followed by in vitro transcription with T7 RNA polymerase (Invitrogen).
The summed images (all timeframes) of the reconstructed data were spatially normalized to an in-house created [11C]raclopride template of the Wistar rat brain.
Preprocessing included correction for slice-timing differences [18], realignment to compensate for bulk head motion, normalization to standard MNI space using an in-house developed EPI template optimized for 7 T EPI imaging.
Laboratories employ different in-house methods for preparation of template DNA as well as different amplification systems.
In CASP8, RAPTOR++ uses three in-house threading methods, a multiple-template method for easy targets and also a template-free method for hard targets.
In addition, participants discussed the possibility of having access to previously funded grant applications to use as a template to guide their own in-house proposal development.
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