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Exact(1)
The first hit is an accurate alignment between the structural query and the structural S1, which just contains two mismatches and two indels in whole alignment.
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Those positions represented by less than 50% of the sequences in the whole alignment are indicated in small letters in the consensus sequence.
The most highly conserved region in the whole alignment is centered on a Cys residue in the motif Cys-Xaa-Gly (bold type indicates residues found in the catalytic triad of sortases).
sequences present in Rfam database (release 11.0) [ 63] were discarded using Bowtie [ 64], allowing one mismatch in the whole alignment region.
The testing showed that there is a little saturation in the whole alignment for each of four analysed nucleotide data sets.
Finally, these quality controlled reads were aligned to the dog genome (Ensemble's CanFam3.1; release 68) using Bowtie, again allowing one mismatch in the whole alignment region.
To assess the impact of compositional heterogeneity, we performed Bowker's tests for symmetry in the evolutionary process on the whole alignment [ 34, 35].
Finally, limitations in whole genome alignment quality may underestimate repeat copy number variation in gene promoters.
Some of the apparent nonalignment of fibrils (resulting in a lower OI) could be due to crimp of the collagen fibrils and not only to the variation in whole fibril alignment.
A straightforward measure is percent identity (in which the number of identical nucleotides in the alignment is calculated as a proportion of whole alignment length).
The knowledge of even one 3D protein structure in a given sequence alignment provides a powerful means to test the correctness of the whole alignment.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com