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Analysis of the suspect regions supported the hypothesis that there were a large number of loci in ES114 in which sequencing errors had led to the miscalling of one gene as multiple ORFs.
The flow cell is a glass slide that contains a lawn of oligonucleotides complementary to the adaptors ligated to transcripts and with a series of separate lanes in which sequencing reactions take place.
It is also reflected in the way medicine is changing in the so-called 'genomic era', in which sequencing each person's genome is becoming a real possibility for routine clinical practice.
Tian et al. (2006) extracted the mitochondrial sequences from a whole-genome shotgun nuclear sequence, in which sequencing was done with MegaBACE 1000 capillary sequencers employing a conventional chain terminating method.
All were sequenced using "traditional" sequencing approaches in which sequencing libraries are constructed from individual segments of the genome (such as those contained within bacterial artificial chromosomes (BAC) clones) and are sequenced via gel electrophoresis and dideoxy terminator chemistry (i.e., "Sanger sequencing").
Adjudicators were unable to determine the linkage status of 3 pairs, described below, in which sequencing was completed (Supplementary Table S1).
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MG: In which sequence?
Parker relies on a pretentious editing scheme in which sequences are bridged with whirling montages of classroom notes — "lust" and "innocence" and "guilt," the notes proclaim.
That freed up time for another round of anguishing meetings about what tracks should make it onto the album, and in which sequence they should be arranged.
For this study, we employed a library preparation method in which sequence reads have barcodes linked to single HMW DNA molecules.
Dancers also turned one another topsy-turvy in "Estuary," a group piece in which sequences in silence were followed by a Tartini sonata for solo violin played by Allen Lieb.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com