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Notably, IGF-IR has also been linked in vitro to development of resistance to tamoxifen [ 55] and trastuzumab [ 56].
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We performed a series of mating experiments to ascertain the number of oocytes ovulated and the potential for embryo development in vitro to the blastocyst stage and in vivo to Day 12 of gestation.
PT was employed as a PhD student at Wageningen University, Department Horticultural Production Chains from 2003 to 2007 and successfully defended his PhD Thesis "Hydraulic properties of Zinnia elegans: from cellular development in vitro to performance in planta" of which this study is a part.
It is therefore imperative to mimic the in vivo tumor niche in vitro to better understand cancer and enhance development of therapeutics.
In addition, we tested the effect of this compound on human osteoclast differentiation in vitro, to explore the possibility of future development of novel RA therapy.
A critical deficit in the use of in vitro differentiation to study early germ cell development is the lack of data to relate requirements in vitro to those in vivo.
Enhanced reprogrammability of nuclear genome descended from TSA-exposed MSCs in the blastomeres of porcine preimplanted cloned embryos resulted in the improvements of not only their capacity to complete in vitro development to the morula and blastocyst stages, but also cytological and molecular quality of the blastocysts produced.
In turn, nuclear-transferred embryos that had originated from porcine undifferentiated bone marrow-retrieved MSCs and their derivatives along the osteogenic lineage were able to complete their in vitro development to blastocyst stage at the rates ranging from 33%to45%5% [ 31].
Treatment of E. invadens trophozoites with TSA or SCFAs blocks their in vitro development to the cyst stage, suggesting a biological role for histone modification in Entamoeba development [ 23].
In addition, the effects of two sperm separation treatments on embryos capable of cleavage and in vitro development to the blastocyst stage were evaluated.
We also previously used this model of in vitro pancreatic development to define signals and conditions that modulate the differentiation of PDX1-positive pancreatic progenitors into NEUROG3-positive endocrine progenitors.
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