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Recovery of betamethasone disodium phosphate from the in vitro test samples was 99.7±5.2% (mean±SD).
The limit of detection for betamethasone disodium phosphate in the in vitro test samples was 0.25 μg.
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For in vitro testing samples, criteria of inclusion for the analysis were (i) monoclonal isolates by using msp1/msp2 genotyping, (ii) wild-type Pfcrt alleles and (iii) successful determination of Pfmdr-1 alleles.
Hereafter, the data analysis was performed only in the 413 samples with valid in vitro test results.
While these tests are relevant to evaluate acute [8] and chronic [9] effects in later life stages, the experimental setup exhibits several in vitro test characteristics, including a reduced volume of sample for testing and potential for high-throughput applications.
In addition, in most studies the water samples have been tested only in in vitro test systems such as the Salmonella-microsome test.
In vitro test using L929 mouse fibroblast revealed that, the number of cells proliferated on PICP-treated samples was higher than that on untreated samples.
The majority of data relating heat treatments to thermal death kinetics has previously been obtained by carrying out in vitro tests on small samples of microorganisms in growth medium or food slurries.
When T cell assays had to be run in batches, a study investigator who was not involved in the in vitro testing selected the samples, such that animals from all groups were included in every run to exclude inter-group bias.
In fact, a human-based in vitro test would have predicted the harmful effects and protected those men (7).
These studies have been experimented upon different benchmark samples including in-vitro test suites and complex assay samples like Bradford protein assay, qPCR, sucrose gradient analysis to test its robustness and the results are really encouraging.
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