Sentence examples for in vitro screen from inspiring English sources
To this end, we performed an in vitro screen using recombinant YAP and a panel of recombinant, active kinases.
These new compounds were evaluated in the National Cancer Institute's 60 human tumor cell line in vitro screen.
As these sensors can be adapted from an in vitro screen to imaging kinase activity in living cells, they support both preliminary and later stages of drug discovery.
The strains expressing xfpk(LM) and xfpk CA), corresponding to the phosphoketolases showing similar activity patterns in the in vitro screen, also showed a similar acetate accumulation pattern with maximal titers of 0.66 ± 0.06 and 0.75 ± 0.06 g/L respectively, whereas the strain expressing xfpk AN) did not show any significant difference in acetate accumulation compared to the control.
The ESK1 gene was initially identified by map-based cloning of the Arabidopsis thaliana esk1-1 mutatiobtainedned using an in vitro screen for constitutive freezing tolerance [12].
To analyze why UFL1 did not bind in our in vitro screen, we used the same GST pull down strategy, using CDK5RAP3-GST as bait.
We have therefore developed and applied an in-vitro screen to triage and prioritize candidate modifier genes for more detailed future studies which is faster, far more cost effective and ethical relative to mouse bioassay models.
Where multiple candidate genes require testing, the constraints of mouse bioassays become prohibitive necessitating the development of a fast, reliable, ethical and cost-effective in-vitro screen to provide a measure of prion-related function to assist with prioritization for in-depth functional genomics.
However, current genome-wide studies are now generating large numbers of candidate modifier genes that cannot be differentiated further by genetic studies and a higher throughput, more cost-effective and ethical in-vitro screen is required to prioritize candidates for in-depth in vivo analysis.
We designed an in vitro screening platform by using late passage (passage 5) of WRN -/- MSCs at low confluence.
