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These collections may not be true representations of TIF due to the ex vivo and in vitro sampling techniques employed.
In vitro sampling showed that 93.02% of components present in the original sample were also found in experimental replicas, with 81.6% of reads found in both.
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First, lncRNA probes were synthesized and labeled with high affinity tags, such as biotin, then cell lysate was prepared from a in vitro sample.
Here, a novel method for the measurement of glycolysis reactants from in vitro samples is presented.
Especially, the in vitro sample contained rather high concentrations of macromolecules and lacked of native membrane environment (Barnes and Pielak, 2011).
In vitro samples were incubated under osteogenic conditions and in vivo samples were implanted subcutaneously into severely compromised immunodeficient mice, for 4 weeks.
Control samples were also used in conjunction with the in vitro samples to take into account an increase in 570-nm photon absorption due to the SGSs themselves, which could obscure correct interpretation of the results.
The smog chamber/in vitro exposure system was designed to investigate the toxicity of chemicals before and after photochemical reactions and to investigate interactions with the urban atmosphere using representative in vitro samples.
The antibacterial assay presented a negative result using in vitro samples and bacteria inhibition with field samples against Staphylococcus aureus (ATCC 29213) and Staphylococcus epidermidis (ATCC 12228) standard strains.
In conclusion, qPCR was a reliable technique to investigate viral load in in vivo and in vitro samples, and EHV-5 replication in equine epithelial cells may be influenced by cellular stages of differentiation.
If a metabolite is only observed in human in vitro samples and is not present in a metabolic pathway defined in the toxicological species already, the toxicological relevance of this metabolite must be evaluated.
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