Sentence examples for in vitro processing from inspiring English sources

In vitro processing of semisynthetic substrates unrelated to the prelacticin 481 peptide demonstrated the broad substrate tolerance of LctM.

This is usually achieved by in vitro processing with the help of an exogenously added protease that cleaves a site introduced between the fusion partners.

MBP-GrB fusion protein was cleaved during secretion by an endogenous furin-like proteolytic activity in vivo, liberating enzymatically active GrB without the need of subsequent in vitro processing.

To circumvent the need for in vitro processing, we included a synthetic furin recognition motif between the protein domains of MBP-GrB fusion proteins and investigated potential in vivo cleavage by endogenous furin-like proteases from yeast.

However, in two other assays, in vitro processing of either fusion proteins or oligopeptides, this peptide proved to be the worst TEVp substrate of all 20 permutationsions tested, except ENLYFQP for which no cleavage could be observed at all [18].

In vitro processing of NCp15 (1 µM) by PR (Figure 1) was studied by examining the effect of several nucleic acids (ssDNA, G4-DNA, RNA and dsDNA) in order to address the positive effect on proteolysis, already shown for RNA [18], [20].

Similarly to what we observed for in vitro-processing of SUMO chains by recombinant Wss1, the isolated WT protein complex accumulated sumoylated species of intermediate size as a result of the proteolysis of HMW-SUMO.

The hypothesis was further supported by other findings 22, 24 in that bevirimat-mediated inhibition of CA-SP1 processing could be reproduced in an in vitro Gag processing assay if purified HIV-1 immature viral core or assembled HIV-1 Gag chimera containing the Mason-Pfizer monkey virus (M-PMV) p12 domain was provided, respectively, as a substrate.

In vitro Dicer processing of pre-miRNAs also show that Dicer processing is generally precise and it cuts ∼22 nt from the Drosha-set end to generate the double-stranded miRNA (ds-miRNA).

The processed transcripts accumulated over the standard in vitro Drosha processing time period of 90 minutes (Fig. 4B, lanes 5, 6, 7), making it unlikely that they resulted from random degradation.

Preliminary results of in vitro enzymatic processing of milled pinewood sawdust with crude MnP concentrates showed up to 17% (data not shown) decrease in Klason lignin content and benefitted from the use of co-oxidants (Tween 80 and glutathione) in some cases.