Sentence examples for in vitro packaged from inspiring English sources

The recombinant cosmid were in vitro packaged and transfected into E. coli XL1-blue MRF' strain.

The ligated DNA products were then in vitro packaged using Gigapack III packaging extract (Stratagene, USA).

The in vitro packaged virions produce distinctly blue plaques when plated on a suitable host.

Insert-DNA was ligated in the pCC1-Fos™ vector, constructs were in vitro packaged into phage particles and transfected into Escherichia coli EPI 300™-T1R.

The double stranded cDNA was uni-directionally ligated to the lambda-ZAP vector, in-vitro packaged and allowed to infect XL1- Blue MRF E.coli cells according to the manufacturer's instructions using a UNI-ZAP XR cDNA library construction kit (Stratagene, La Jola, California).

Nevertheless, T7 and λ systems rely on in vitro packaging systems that are quite labile and have limitations for the construction of libraries with large number of variants with a limit up to 107 to 108 variants.

Plasmid pGL3-control (Promega cat # E1611), which carries the luc reporter gene expressed from the early SV40 promoter, was used for the in vitro packaging experiments.

The ligation reaction was incubated at 16°C, subjected to in vitro packaging, and titered to determine the number of plaque forming units (pfu).

After in vitro packaging, library containing 2×10 primary cDNA clones was obtained.

The obtained DNA fragments were inserted randomly into the cosmid vector Charomid 9-28 (Sando and Stark 1986) after digestion with BamHI and were then subjected to in vitro packaging using a LAMBDA INN in vitro packaging kit (Nippon Gene).

Libraries with diversities in the range of 10 10 are constructed using commercially available in vitro packaging systems.