Sentence examples for in vitro modifications from inspiring English sources

In this context, recombinant proteins can be bioproduced on a large scale, without the need for further in vitro modifications, being free of known or suspected biohazards.

The method designed by Folch [35] was originally designed for the extraction of hydrophobic substances, but this method is also useful for extracting low molecular weight hydrophilic organic compounds, essentially without any in vitro modifications, i.e., oxidation hydrolysis and decomposition.

Previous reports of in vitro modifications of bovine serum albumin (BSA) by methylglyoxal [15] and glucose [16] indicate that these modifiers decrease content of the sulphydryl groups, impairing the antioxidant properties of albumin and inducing conformational changes attributed to diminishing of the fluorescence of the tryptophan residues.

(b) In vitro modification assay of BioID and PUP-IT to compare the activity.

Most published cloning systems require in vitro modification of the insert DNA and the vector DNA with techniques beyond a simple PCR.

Although reported here in a DIPG sample for the first time, systematic in vitro modification of the lysine 27 predicted K27I as the only substitution other than K27M to result in a repressive effect on H3K27me3 [ 34].

In order to further reduce the complexity of the experimental system, we used an N-terminal fragment of PARP-1 that is liberated upon caspase cleavage in vivo (aa 1 214) as a substrate for in vitro modification assays.

Bacterial transglutaminases are increasingly required as industrial reagents for in vitro modification of proteins in different fields such as in food processing as well as for enzymatic site-specific covalent conjugation of therapeutic proteins to polyethylene glycol to get derivatives with improved clinical performances.

In vitro-translated, full-length F-NACC1 generated with the wheat germ extract system and recombinant His-SUMO proteins were subjected to an in vitro modification reaction by incubation with a mix containing recombinant Aos1/uba2, recombinant Ubc9, and ATP in either the presence or the absence of recombinant SUMO.

Aim: A biomechanical study was conducted to determine the in-vitro modification of the posterior cruciate ligament tension following different types of total knee arthroplasty (TKA).

However, the knockin efficiencies reported so far have not allowed more complex in vitro genome modifications such as, for instance, simultaneous integration of a DNA fragment at two distinct genomic sites.