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Thus, we developed an assay in which in vitro maturing pig ova are incubated with purified, heat-activated sperm proteasomes during the last four hours of in vitro maturation (total 44 h of IVM).
This coincubation of in vitro maturing ova with purified, heat-activated sperm proteasomes caused zona digestion, resulting in a striking abrasion and loosening of the zona (Fig. 8 a-d).
In fact, we found that exposure of in vitro maturing DCs to spermine enhanced the expression of CD83, suggesting that this tumour-derived polyamine could be involved in the phenotypic maturation of PB DCs observed in vivo.
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It is interesting that in vivo matured MII and in vitro matured MII oocytes shared very similar patterns of gene expressions, but they also noted some significant differences (see Table 6).
To develop and test a simple, feasible approach to improve spindle assembly and developmental competence of human in vitro matured oocytes by parthenogenetic activation in maturation medium.
It was found that GV oocytes expressed 12,219 genes, in vivo matured MII oocytes 9,735, and in vitro matured MII oocytes 8,510.
Concisely, prior to the SCNT procedure, cumulus-denuded in vitro matured gilt/sow oocytes were incubated in the maturation medium supplemented with 0.4 μg/mL demecolcine (DMCC) for 50 to 60 min.
Aim: To determine whether maturation and subsequent blastocyst development of in vitro matured oocytes can be improved by in vivo follicle stimulating hormone (FSH) or human chorionic gonadotrophin (hCG) priming, using a mouse model.
Although the in vitro matured MII oocytes closely resemble the in vivo matured oocytes, especially for genes related to nuclear maturity, there were several genes related to cytoplasmic functions which were expressed in an immature manner.
To describe the possible effects of cryotop vitrification on maturation rate and ultrastructural morphology of human in vitro matured germinal vesicle (GV) oocytes.
In summary, we show new original data obtained by genome wide analysis of in vitro matured human oocytes and embryos, revealing the almost autonomous maturation of human oocytes and early embryogenesis.
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