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These effects, most easily studied in vitro, include DNA double-strand breaks, chromosomal aberrations, gene mutations and cell transformation.
Models used for culture of Cryptosporidium in vitro include Madin-Darby Canine Kidney (MDCK) cells and the human cell line HCT-8 cells.
Culture methods for the enhancement of isolated primary hepatocytes or HLCs in vitro include the addition of GFs, TFs, and cytokines, the activation of signaling pathways, the use of an optimized matrix, and co-culture with various nonparenchymal cells.
The principal mediators of EBV-induced growth and cellular transformation of B lymphocytes in vitro include EBNA2, EBNA3A, 3C and LMP1 proteins [11].
Endothelial cell responses to Ang1 in vitro include chemotaxis, prevention of apoptosis, sprouting, and tube formation.
The isolation methods of EPCs in vitro include flow cytometry, MASC (Magnetic Activated Cell Sorting) and density centrifugation.
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Currently, methods of sterilization described either in-vivo or in-vitro, include autoclaving [ 4, 5], pasteurization [ 6- 8], extracorporeal irradiation [ 9- 12], microwave [ 13], and liquid nitrogen [ 14].
The characterisation in vitro included stability studies in different media and determination of logD (octanol/PBS).
Characterization of compounds in vitro (including 9i; Mled1) led to the determination of key structural requirements for BIR2 binding affinity.
In recent years, a variety of organoids have been cultured successfully in vitro, including the stomach, liver, kidney, lung, gut, brain, and retina (Clevers, 2016).
The effects of polysaccharides on plasma coagulation parameters in vitro including APTT, PT, TT and FIB were assayed and the results were described as follows.
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