Sentence examples for in vitro evolving from inspiring English sources

Following this combined approach, the first mutation-accumulation experiment with in vitro evolving populations of RNA molecules was recently performed [ 36].

But extension of an in vitro evolved pathway of the fully desaturated 2,4,2′,4′-tetradehydrolycopene 2,4,2′,4′-tetradehydrolycopenel fully desaturated C40 dialdehyde carotenoid 2,4,2′,4′-tetradehydrolycoproduced

Surprisingly, in the in vitro evolved complex one of the partners is rotated 180° relative to the original design model, yet still maintains the central computationally designed hotspot interaction and preserves the character of many peripheral interactions.

We identified two positions with characteristic differences between naturally and in vitro evolved preferences.

The in vitro evolved Val-5 was replaced with a glycine (Gly-5) to assess side chain function.

Using the in vitro evolved sequence pattern we performed a bioinformatics analysis to identify potential novel DYNLL-binding proteins.

In Figure 2 the logo of the in vitro evolved pattern is compared to the logo of 41 known naturally evolved DYNNL1 binding peptides (listed in Table S2).

In case of the naturally evolved consensus the improvement was 80-fold, while for the in vitro evolved consensus it was over 500-fold.

Binding parameters of the in vitro evolved Ac-VSRGTQTE and naturally evolved Ac-DKSTQTD consensus peptides were determined by isothermal titration calorimetry (ITC).

Ac-5 versions of both the natural and the in vitro evolved consensus peptide had binding affinities in the micromolar range.

Based on the in vitro evolved sequence pattern we predict a large number of novel DYNLL binding partners in the human proteome.