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Cesarman, E. et al. In vitro establishment and characterization of two acquired immunodeficiency syndrome-related lymphoma cell lines (BC-1 and BC-2) containing Kaposi's sarcoma-associated herpesvirus-like (KSHV) DNA sequences.
Verena Charwat's main research interest is in the field of biomedical engineering, with a special focus on the development and application of lab-on-a-chip systems with integrated biosensors for in vitro establishment and non-invasive monitoring of functional tissue mimics.
In conclusion, we report here in vitro establishment of two EBV-infected NKL sublines, TL1 and TL2.
In vitro establishment is a rare event, found in no more than 1% of primary cancers [ 26] and almost certainly involving further selection of an 'establishment' phenotype.
The high scores for p53 and Ras likely reflect the exceptional selective advantage of mutations in these two genes for tumor growth and during in vitro establishment of cell line cultures.
In conclusion, it is now very obvious that the in vitro establishment of long-life fully functional hepatocytes for BAL development, drug discovery and toxicity testing or even transplantation depends on several factors that need to be integrated into in vitro models.
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Immature inflorescences are a significant resource for in vitro culture establishment.
The transcription factor Oct4 is required in vitro for establishment and maintenance of embryonic stem cells and for reprogramming somatic cells to pluripotency.
These include the initial extension of multiple immature neurites (days in vitro, DIV1-2), establishment of axon dendrite polarity (DIV2-4), andn andendritete outgrowth (DIV4-15), and finally synapse formation and maturation (after DIV10).
It should be underlined that our reference ELP-MLP condition (35 μM Pi in the root culture medium and 0 μM in the spore germination medium) corresponds to the standard growth conditions that were found to be optimal for in vitro AM establishment [ 37, 38].
Table 1: In vitro development and establishment of ntES cell lines from urine-derived cells.
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