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Bradbury, A. R., Sidhu, S., Dübel, S. & McCafferty, J. Beyond natural antibodies: the power of in vitro display technologies.
Bradbury, A. R., Sidhu, S., Dubel, S. & McCafferty, J. Beyond natural antibodies: the power of in vitro display technologies.
Single chain variable fragments of antibodies (scFvs) provide binding specificity and offer an increased ease of in vitro display selection.
Also discussed is an emerging new direction for in vitro display technology: the self-assembly of protein arrays.
Here, we review recent conceptual advances that now enable in vitro display of multimeric proteins, integral membrane proteins, and proteins with an expanded amino acid repertoire.
The in vitro display on phage T4 offers a novel platform for potential construction of customized vaccines against anthrax and other infectious diseases.
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In-vitro display technologies combine two important advantages for identifying and optimizing ligands by evolutionary strategies.
We also find that although the isolated spinal cord in vitro displays spontaneous bursting activities in sensory and motor zones, their spatiotemporal dynamics poorly matches that of the spinal cord in vivo, even after deafferentation.
6-OHDA-induced toxicity in vitro displays differences among the brain structures, but it is also observed in cerebrocortical and hippocampal slices, besides striatum.
ESCs cultured on elastic polymer [poly(lactide-co-caprolactone), PLC L]scaffolds subjected to cyclic strain in vitro displayed elevated cardiac gene expression compared to unstrained controls.
The HR212 or GST-HR212 protein, which binds the HR1 peptide in vitro, displayed inhibitory activity against NDV-mediated cell fusion, while the HR121 and 5-Helix proteins, which bind the HR2 peptide in vitro, inhibited virus fusion from the avirulent NDV strain when added before the cleavage of the fusion protein.
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