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To compare directly in vitro developmental competence between parthenogenetically activated and intracytoplasmic sperm injection (ICSI -fertilized oocytes.
To study the effect of human embryo multinucleation on the rate of aneuploidy, in vitro developmental morphokinetics, and pregnancy outcome.
We hypothesized that the proportion of oocytes demonstrating a normal spindle apparatus and in vitro developmental potential would be proportional to the solution osmolality.
Establishing a reliable in vitro developmental model using human ESCs will be critical for further elucidating the specific and overlapping roles of Mesp1 and Mesp2 in early mesodermal development of humans.
To assess the efficacy of human embryo twinning by blastomere biopsy at different early embryonic stages (splitting efficiency) and to determine the in vitro developmental capacity of twinned human embryos (developmental efficiency).
Although the in vitro developmental rate was not improved, the abnormal H3K9me2 level was corrected in SCNT embryos from UNC0638-treated GFFs (T-SCNT) compared with C-SCNT embryos.
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Each stage was characterized as an established in vitro ESC developmental process of chondrogenesis.
It is interesting to note that while PI3Kβ−/− and PLCγ2−/− mice had similar increases in trabecular bone mass, PLCγ2−/− mouse cells showed a much more robust in vitro osteoclast developmental defect than PI3Kβ−/− mouse cells.
To provide a comprehensive overview of Arabidopsis SE at a transcriptional level, we sequenced cDNA libraries constructed from three distinct in vitro embryo developmental time-points using the Illumina HiSeq 2000 platform.
Toxicogenomic evaluations may improve toxicity prediction of in vitro-based developmental models, such as whole embryo culture (WEC) and embryonic stem cells (ESC), by providing a robust mechanistic marker which can be linked with responses associated with developmental toxicity in vivo.
Next, oocytes were fertilized and cultured in vitro to assess developmental rates.
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