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Our results indicate the in vitro conversion assay used in our study does mimic the species barrier of mice to the TSE agents that we tested.
We have successfully established primary 3D organoid cultures of diverse tissues and used them to achieve the first in vitro conversion of primary intestine, stomach and pancreas tissue to adenocarcinoma (Ootani et al, Nat Med 2009; Li et al, Nat Med 2014) amongst others.
Extensive in vitro conversion of complement components C3 and C4 has been observed in EDTA plasma obtained from a number of stable orthotopic liver transplant recipients (LTR) [Clin. Chem. 45 (1999 11900].
E. coli cells expressing the PPAT(M2T/Y35H/V70K/E212G) could be used for in vitro conversion of pyridoxal into pyridoxamine at 30 °C with l-glutamate as an amino donor.
Transmission was simulated by fibrillzation in our in vitro conversion assay with the exact concentrations of that particular species.
To further confirm the specificity of inhibition by the inhibitor SB203580 and the control inhibitors of JNK (SP600125) and ERK (PD98059), we analysed the activities of the p38, JNK and ERK pathways during in vitro conversion into iTreg (Figure 5A).
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The multiscale approach to design a model of TMZ intracellular brain disposition progressed through the following steps: (i) TMZ pH-dependent conversion study in solutions, (ii) in vitro TMZ conversion and transport studies in U87 glioma cells, (iii) TMZ PK studies in mice, and (iv) TMZ PK studies in brain tumor patients.
The procedure described by Prem et al. [ 29] was followed for in-vitro embryo conversion to plantlets (germination).
First, we tested NOS activity in homogenates made from MCF-7 cells treated with 10 μM rifampicin or solvent control for 24 and 48 hours by measuring in-vitro biochemical conversion of C L-arginine into C L-citrulline as a direct measure for iNOS activity [ 59].
Purification and in vitro enzymatic conversion by peptidylglycine α-amidating monooxygenase yields authentic, biologically active salmon calcitonin.
Although there is not a perfect correlation between data obtained from the cell-based in vivo fluorescence assay and the in vitro substrate conversion experiment, importantly, the hierarchical order of the substrate processing efficiency was identical in either context.
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