The ability to direct recombination between DNA sequences in vitro constitutes the basis of modern molecular genetics.
These data support the idea that the interaction we identified in vitro constitutes the primary molecular determinant of the effects on VGCCs seen in Munc13-KD neurons.
To equate such low-intensity microglial activation in human brain with artificially produced high-intensity microglial neurotoxicity in vitro constitutes a flaw in scientific reasoning explained possibly by immature judgment or premature ambition.
Human adipose-derived stromal cells (hASCs) have been shown to be easily isolated, expanded, and differentiated into hECs in vitro, constituting an excellent option for large-scale production of compatible and functional hECs.
For control experiments, BTO dimers in vitro, constituting probe-strand-conjugated BTO NCs and capture-strand-conjugated BTO NCs, were mixed in equal proportions, and then the target strand was added in lower concentration than the conjugated BTO NCs (2 1); the mixture was kept at a temperature of 60 °C, which was lower than the melting point of the DNA hybridization.
Using an in vitro model constituted by human LoVo cells, we measured the enzymatic activity of the recombinant yCD protein in converting the antifungal agent 5-FC into the highly toxic anticancer compound 5-FU.
Patients with a low lying placenta, particularly if the placental margin appears at the internal os, and pregnancies conceived following in-vitro-fertilization constitute 'at risk' groups as well.
Instead, it was an "in vitro" evaluation, and this constitutes one of the limitations of the study.
Our novel in vitro test constitutes a simple, rapid and cheap functional assay to evaluate sperm chromatin decondensation ability.
The transfer of cells from a stem cell niche to an in vitro culture constitutes a drastic change in environmental conditions leading to a complete reorganization of the stem cell population.
