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Replicative senescence of primary fibroblasts in vitro consists in the progressive loss of cell division abilities with cellular morphological changes and the accumulation of senescence-associated β-galactosidase activity [ 33].
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We have developed a method of forming biphasic constructs ("osteochondral -type plug) in vitrosteochondral -typetilaginous tissue, formed on and anchored to the intended articulation surface of a plugus ceramin substrate.
Antigenic stimuli used in vitro consisted of a pool of 57 peptides spanning TRAP and 48 peptides comprising CS (20mers overlapping by 10aa).
Synovial fluid adherent cells in vitro consist of two subpopulations, large round-shaped macrophage-like cells(CD68+) and spindle-shaped fibroblast-like cells (Thy-1+).
To examine the ability of S. oralis to stimulate this receptor we first utilized an in vitro reporter system consisting of HEK293 cells stably expressing TLR2, with a chemokine (IL-8) secretory response as a readout.
The in vitro study consists of the characterisation and detection of inflammatory markers on activated endothelial cells by immunocytochemistry and MRI using biotinylated anti-P-selectin and anti-VCAM-1 (vascular cell adhesion molecule 1) antibody and streptavidin conjugated SPIO.
Apart from the fact that the in vitro culture consists of a single cell type growing on a plastic surface, the soluble factors are different.
It is likely that the CK18 Asp NE standard used in the present work, which was generated by the induction of apoptosis in cancer cells in vitro, also consists of aggregates.
Our in vitro model consists of a three-dimensional (3D) direct co-culture system in Matrigel® similar to that utilized by Debnath and colleagues and by Shekhar and colleagues [ 19, 22].
The in-vitro model consisted of one fresh-frozen cadaveric radius (age: 62, male) and an interposed modular axial load transducer device (Fig. 1).
To address this hypothesis, we utilized an in vitro model consisting of murine ESCs-derived embryoid bodies (EBs).
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