Overexpression of CBX6 inhibited the proliferation and metastasis capacity in breast cancer cells in vitro, altered the expression of genes involved in cell cycle regulation and other pathways.
Wild-type, but not Crohn's-associated, Nod2 LRR domains directly interacted with bacteria in vitro, altered their metabolism and disrupted the integrity of the plasma membrane.
We found that these mice display abnormal synapse organization and maturation in vitro, altered synapse density in the adult brain, enhanced glutamatergic transmission and reduced synaptic plasticity in CA1 hippocampus.
Finally, this regimen of hypoxia conditioning in vitro altered the angiogenesis activity of the resulting tumours grown in vivo.
MCF7 breast tumor cells engineered to express high levels of murine podocalyxin (MCF7Podxl) exhibit increased migration in vitro, altered morphogenesis and disrupted cell cell and cell matrix contacts [ 10, 19, 20].
Therefore, the reduction of macrogametes in vitro, altered sex allocation, and decreased infectivity in Δ ppm2 parasites could suggest that the protein is vital for the response of Plasmodium parasites to environmental factors, or that longevity of the mutant female gametocytes is affected, resulting in lower numbers.
While fluorescence and lifetime measurements of CPMV and TMV with less dye exhibit enhanced fluorescence properties, this may not translate into cell imaging studies in vitro; altered cell uptake properties and chemical degradation of the optical within the endolysosome results in brighter signals from particles with increased dye loading.
For instance, it was recently observed [ 121] that the incorporation of DHA in immortalized colonocytes in vitro altered the lateral organization of the Epidermal Growth Factor Receptor (EGFR), thus leading to increased ligand-induced receptor dimerization and phosphorylation along with its internalization and degradation.
Cytoskeletal disruption in vitro alters Na channel kinetics, producing a late Na current that can prolong repolarization.
It would be interesting to investigate whether a change in input to the network in vitro alters the distribution in a similar way to those distributions seen in Fig. 9.
