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On average, MSCs from ICBM aspirates and LBFBM aspirates have both undergone 16PDs, with no apparent correlations being found between the "in vitro age" and functional outcomes for individual cultures.
We next investigated whether any observed donor-to-donor differences could be attributed to the "in vitro age" of tested cultures (measured as total PDs at P3, i.e. prior to differentiation).
In a screening of a large chemical library of ca. 1,300 compounds, edaravone, a drug used to treat cerebral infarction, was discovered that had in vitro AGE inhibitory activity (Tsujita et al. 2004).
Proteoglycan synthesis was within normal value in basal medium; however, in this culture condition, comparisons are difficult and it has been previously reported that proteoglycan synthesis varies greatly among cultures depending on tissue source of the cells, the in vitro age of the culture and as yet unidentified factors [Harper et al., 1987].
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The optical properties of orthodontic aligners appear to vary between brands and constituent materials but deteriorate with in vitro aging in all cases.
This is in line with findings by Gracco et al. that both in vitro aging (artificial saliva) and in vivo wear increase the absorbance after 14 days.
The aim was to assess and compare absorbance and transmittance values of three types of clear orthodontic aligners before and after two cycles of in vitro aging.
For grafts having lower relative porosity (35 45%), solubility plays a more crucial role in mass loss during in vitro ageing and in vivo resorption.
This leads to in vitro aging, the accumulation of intracellular oxidative damage, and subsequently a decreased potential for proliferation and differentiation.
As few in vitro studies on this cement system have been performed, this study aimed to compare the effects of static and dynamic in vitro ageing protocols on the phase composition, weight loss and mechanical properties of brushite cement.
To further advance the knowledge in this field, hMSCs were cultured and routinely passaged on tissue-culture polystyrene to investigate the correlation between cell stiffening and differentiation potency during in vitro aging.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com