Indeed, a search of miRNA seed sequences reveals several miRNAs that can potentially recognize AUG codons in various nucleotide contexts, raising the possibility that particular miRNAs affect the translation of some mRNAs by masking start sites.
DOI: http://dx.doi.org/10.7554/eLife.04686.021 Comparison of kinesin's active site in various nucleotide states indicates that there are two ways to keep the nucleotide cleft closed, depending on the conformation of the switch loops.
Crystal structures of isolated DnaK NBDs in various nucleotide states have failed to capture the delicate intersubdomain allostery that modulates substrate binding affinity, whereas solution-state NMR spectroscopic studies have instead demonstrated that ATP or ADP binding significantly alters NBD subdomain orientation and flexibility.
(A) MamK polymerization in the presence of various nucleotide states was assessed by a pelleting assay.
Over the last decade, mice with defects in these various nucleotide-sensing TLRs or their downstream adaptor molecules have been shown to exhibit diverse outcomes when challenged with different viral pathogens (Akira et al., 2006; Zhang et al., 2007).
The frequency analysis of various nucleotide repeats in C. sinensis ESTs revealed that di nucleotide SSRs were the most abundant SSRs followed by tri-, tetra-, penta- and hexa repeats.
The crystal structures of the GTPase domain of rat dynamin 1 (Reubold et al., 2005), and the GTPase domain of Dictyostelium dynamin A (Niemann et al., 2001) and of the dynamin-related guanylate-binding protein 1 (GBPrakashakash et al., 2000) in various different nucleotide states (Ghosh et al., 2006), have been solved.
Open image in new window Figure 2 Catalytic efficiencies and reaction processes of MamK towards various nucleotide states.
Various nucleotide states, including ATP, ADP, AMP, GTP, GDP and GMP (Sigma, USA), were used as standards to determine the retention times and amounts of the various ingredients in the reaction mixture.
As shown in Figure 2A, various nucleotides and nucleosides such as AMP, UMP, cytidine, inosine, hypoxanthine, and uridine were present in the FasL-treated cell supernatant.
In this assay, various nucleotides at a concentration of 1 mM were added individually to GsuK-containing liposomes loaded with high [K+] followed by mixing with the flux buffer containing radioactive Rb.
