The following settings were used for the database search: trypsin-specific digestion with one missed cleavage allowed, carbamidomethylated cysteine set as fixed modification, oxidized methionine and deamidation in variable mode, peptide tolerance of 60 ppm and fragment tolerance of 0.1 Da.
In our own species, variable modes of carrying out social interactions between different communities are often imbued with a deeper significance; and it is this added layer of meaning that makes these acts truly cultural, shared collectively by a community as whole [22].
Since the plaid models are only created in the variable mode (which is always the case for IDR), the object mode is not shown.
The AED operating mode, either "manual" or "advisory," was coded in the variable "mode".
Studies confirming the expression of ANP and its receptors and their variable modes of regulation in the immune system [30] support the notion that ANP has immunomodulatory potency.
The 2-D gels were scanned in a Typhoon 8610 variable mode imager (GE Healthcare) at 300 dpi resolution and gel images analysed with Melanie software version 7.0 (Geneva Bioinformatics (GeneBio) SA).
When necessary, the separated H-labelled steroids were visualized by exposing TLC plates to Fujifilm BAS-TR2040S imaging plates (Raytek Scientific Ltd., Sheffield, UK), which were then scanned in a Typhoon 9410 variable mode phosphorimager (GE Healthcare, Amersham, UK).
Labelled proteins were visualised using a Typhoon™ 9400, Variable Mode Imager, (GE Healthcare), scanned in-between low fluorescence glass-plates (GE Healthcare).
These will be referred to as Variable Mode Regions (VMR) in the following.
After the run was completed, the 2-D DIGE gels were scanned in situ using a Typhoon 9410 Variable Mode Imager (GE Healthcare) according to the manufacturer's instructions.
