Sentence examples for in two replicas from inspiring English sources

In order to assess specificity of commercial reagents used to detect IgA and IgG in the sandwich ELISA assay, a preliminary experiment of cross-detection was performed in two replicas.

Four hybridizations in two replicas were carried out for each time interval (8 and 24 h) for CRC cell lines HCT116 and HT29.

Since we expected that the same CGI be methylated to the same extent in two replicas of the same cell line, a reasonable hypothesis to explain the presence of IRDM-CGIs is that they occur by chance.

Each experiment was performed in three replicas.

For each serotype, the growth experiment was performed in four replicas on different days.

Each experiment was done in quadruplicate for the PI assay and in three replicas - for the Annexin V test.

The LC/MS analysis was performed at least twice for each extract resulting in nine replicas for each condition.

ELISA assays were performed coating 96-well microplates with 50 μg of total protein extract for each sample in three replicas.

For quantification of ARR22 transcript, RNA and cDNA was prepared as described above with addition of random primers for reverse transcription and used as a template for qRT-PCR with Platinum Quantitative PCR SuperMix-UDG (Invitrogen) in three replicas.

The siliques were collected in five replicas for each line at the developmental stage, where the first senescing siliques appeared on the inflorescences, frozen in liquid nitrogen, homogenized and lyophilized.

For fertilization, 3 × 33 µl aliquots of the conidial suspensions were inoculated across the surface of a female tester (in three replicas, each covering roughly the same amount of area).