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We expressed and purified human Hsp70 in two expression systems: Sf9 insect cells and E. coli.
LCN2 staining was not detected at significant levels in the ovarian cancer cohort or in the epithelium of normal tissues, despite expression being increased in ovarian tumors in two expression array analyses [15], [18].
In this work, the recombinant active Caspase 3 and artificial miRNAs were put in two expression cassettes separately with recombinant AFP enhancer/promoter and transcription termination signal bovine growth hormone polyadenylation (BGH poly A).
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This form includes two characters, 文 wén (in two expressions, 天文学 tiānwénxué 'astronomy' and 古文明 gǔ wénmíng 'ancient civilization') and 眼 yǎn (in three expressions, 眼镜蛇 yǎnjìngshé 'cobra', 眼中钉 yǎn zhōng dīng 'nail in the eye', and 小心眼 xiǎoxīnyǎn 'narrow-minded').
With a voice that curls at the edges in contempt and a face that holds a far-reaching scale of ambivalence in one expression, he commands our attention through what is largely a sustained monologue.
The normalized values for each peptide and slide were stored in one expression matrix for each group.
Given the drastic reduction in GR expression at both the mRNA and protein levels and the absence of defective allele expression, we suspected that the mutated GR protein was not expressed in vivo.
According to our experimental design, all the 60-mer probes (except for those redundant ones) were tested on four arrays, resulting in eight expression measurements as each array was scanned at two PMT gain settings.
The 12-point scale was categorized in three expression groups: 0 = no expression; 1 5 = weak expression; 6 12 = high expression.
The elevated expression of SYCP2 in HPV‐associated tumor tissue has previously been noted in three expression analysis studies.
As shown in Figure 4, domestication and crop enhancement over 5000 years has resulted in variable numbers of genes in eight expression profiles in wild cotton becoming distributed in domesticated cotton into the expression patterns defined by PAM clustering.
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