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Each test was run in triplicate to verify the reproducibility of the data.
Each experiment was performed in triplicate to verify results.
All experiments were done in triplicate to verify the results.
Samples with artefacts were typically evaluated in triplicate to verify the result.
Each PCR amplification was performed in triplicate to verify the results.
Each sample was processed in triplicate to verify the specificity of the amplification reaction.
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Two groups of positive control probes were designed: small RNA synthetic spikes were added to the RNA before labeling to verify the labeling efficiency, and probes for abundant small RNA (e.g., small nuclear RNAs U43, U49, U24, Z30, U6, U48, and U44, and 5.8s and 5s ribosomal RNA) were spotted on the array in triplicates to verify RNA quality.
Each sample was tested at least four times in triplicates to verify the repeatability of the assay.
All western blot analysis was conducted in, at least, triplicates to verify accuracy.
50 μL of RPMI was added in triplicate to correct for background.
All measurements were performed in triplicate to ensure high quality data collection.
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