Sentence examples for in triplicate to make from inspiring English sources

PCR efficiency was tested for each primer pair by 10-fold dilution series of cDNA in triplicate to make sure that efficiency is appropriate for the 2-ΔΔCt Pfaffl et al. method [ 28].

Following overnight incubation for adherence, 100 μl cell growth media or varying dilutions of frankincense oil (at1 600 to 1 4,000 final concentration) in their growth media were added to each well in triplicate to make a total of 200 μl.

All of the samples were analyzed in triplicate in order to make the data reliable for statistical analysis and to increase protein coverage using stringent protein identification criteria.

We analyzed all samples in triplicate and made relative comparison to adult testis control cDNA using the ddCt method.

We used the PTR-MS to make real-time measurements in triplicate of six gas-phase VOCs in the machine-generated mainstream smoke on a puff-by-puff basis.

We used a second array consisting of 1024 peptides based on known phosphorylated motifs in human proteins found in the Human Protein Reference Database (HPRD) [36] spotted in triplicate again made available by Pepscan Systems Briefly, a panel of known, phosphorylation motifs derived from different signalling cascades were selected from the proteins annotated in HPRD.

Slides containing ~3,500 BACs in triplicate were made at the Leiden University Medical Centre.

In triplicate observations made 1 and 2 days after Caki-1 and 786-O cells were treated with 5  μ M WP1066, it was found that the total number of WP1066-treated cells was significantly (P<0.01) less than the number of DMSO-treated cells.

In the first instance, the EphB4 gene and the house-keeping control gene PBGD were amplified in triplicate from cDNA made using three different RNA extractions for each cell line and a serial dilution of one of these cDNA preparations using a BioRad iCycler MyiQ.

Serial dilutions of proteins (in triplicates) were made in buffer F (20 mM HEPES pH 6.8, 150 mM KCl, 1 mM EDTA), and 20 nM (wild-type primase and ΔCTD-primase) or 80 nM (PriL-CTD) of fluorescein-labeled DNA oligos.

For each sample, the Ct (Cycle threshold) was used to determine the relative amount of target gene; each measurement was made in triplicate, and normalized to the reference gene b- Glu, which was also measured in triplicate.