Noting the rebound in transcript at 96 h, we further investigated whether repression of HS biosynthesis along the basal lamina of the salivary gland distal lateral lobes was lost at 96 h post-dsRNA delivery.
In fact, among the 4576 biding sites identified in the 5'-UTR region of 2550 genes, 546 are absent in one or more strains at 6 g/l, while 531 are absent in transcripts identified at 45 g/l.
DOI: http://dx.doi.org/10.7554/eLife.03523.014 10.7554/eLiFigure23.015 figure 5—figure supplement 4. Translational efficiency in transcripts expressed at different levels.
In transcripts initiated at iTSSs, the first encountered ATGs are most of the time out-of-frame relative to the main ORF.
In particular down-regulation of the transcripts involved in cell development and differentiation at the early stages of transformation, induction of protein biosynthetic pathways, alteration in embryonic antigen expression and in apoptotic transcripts at the latter stages of transformation.
Terms that indicate metabolic processes, including lipid and carbohydrate metabolism were enriched in downregulated transcripts at both time points and in both species.
We also witnessed a transient reduction in ATOH8 transcripts at T24, which could be attributed to the change in the culture media (growth medium to differentiation medium).
However, we did not observe an early increase in Ig transcripts at the target site.
We observed a decrease in Mbp transcripts at 48 h after treatment with Wnt3a and BMP4, mirroring our IHC results.
You'll be handing in your transcripts at the end of these courses to apply for a license, so get good grades!
