In addition, multiple scattering components were again included in the evaluation in order to check if this part is correctly segmented as background.
In the homogeneous environment, in order to check if our distribution of woodlice in each experiment corresponded to aggregation, we analysed radial and angular distributions of woodlice.
In order to check if F255 in TM6 actually interacts with V296 in TM7, we investigated a double cross-mutant, F255V/V296F, which is expected to recover the original interaction between both positions.
The key questions included in the scenario were previewed in advance in a group of 4 FP/GPs in Poland to check if they could be readily understood.
In order to check if perturbation in inner membrane integrity is accompanied by loss of viability, live dead staining and in parallel plating of treated cells were carried out.
In that case, additional testing is required in order to check if that artificial selection would cause any bias in other properties of the samples.
In order to check if the reduction in soluble Cul1 was also due to its recruitment to aggregates, immunohistochemistry was done on mice brain sections using Cul1 antibody.
