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The N-methyl-1,4-dihydronicotinate ester of an alkylating nitrogen mustard NM-CDS (4) was synthesized in three step reactions.
In this work, three different analogues of AAZTA bearing one or two phosphonic acid residues were synthesized in three step procedures and moderate to good yields.
Thus, overall PT kinetics at different temperature range is well understood by invoking in three step mechanism: I) early stage anatase-to-rutile transformation is dominated by interface-nucleation, II) then intermediate stage reversible rutile-to-anatase PT and, III) at later stages, anatase-to-rutile PT is controlled by dissolution precipitation mechanism.
The percentage of positive cells for p-STAT3 was classified as 0 (none), 1 (≤50 %), 2 (50 to 90%%), and 3 (> 90%%) and staining intensity was graded in three step scale, as follows: 1 (low), 2 (medium) and 3 (high) intensity.
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