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An electrophoretic mobility-shift assay and Ku antisera were used to show that Ku or a closely related protein was deficient in three mutant hamster cell lines from x-ray-sensitive complementation group 5, which is characterized by defects in DNA double-strand break repair and V(D J recombination.
In three mutant OHCs, bundle displacements were measured and it was found that a bundle displacement of 57±16 nm (in the negative direction) was required to elicit a transducer current at −84 mV.
Fig. 3 shows examples of wheel running activity during the first hour of the night in three mutant mouse strains with previously recorded motor deficits.
Initial characterization revealed peri- and postnatal lethal phenotypes in three mutant strains (Fendrr, Peril, and Mdgt), the latter two exhibiting incomplete penetrance and growth defects in survivors.
Furthermore, we determined whether the tests could be used to monitor the onset and progression of motor dysfunction in three mutant strains with distinct aetiologies.
To determine whether H3K36me3 has a direct role in the recruitment/activity of DNMT3b in vivo, we measured the DNA methylation distribution in three mutant strains: set1Δ, set2Δ, and dot1Δ (Supplementary file 1E).
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To identify the mechanism of action of CIN, we examined the effects of CIN on life span in three mutants implicated in major longevity pathways.
In most cases, SlCaMs in three mutants had higher expression than those in wild-type.
Lutein was not altered in LL and only slightly higher in three mutants in HL conditions compared to the wild-type strain.
The percentage of 1N1K cells was increased in only two mutant cell lines (T275 and T305), and similar to the reference cell line in three mutants (T273, T291, T325), but lowered in the 34 others.
Quantification of TAGs on a cell basis in three mutants affected in starch synthesis (cw15sta1-2, cw15sta6 and cw15sta7-1) showed that blocking starch synthesis did not result in TAG over-accumulation compared to their direct progenitor, the arginine auxotroph strain 330.
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CEO of Professional Science Editing for Scientists @ prosciediting.com