Exact(12)
The third song in "Three Fragments" is a winding fugue played by the ensemble (flute, guitar, violin and cello) while the singer intoning the love poem is separate, "locked out," from participation in the fugue.
Amplification using ITS1 and ITS4 conserved region primers gave an amplicon of 607 bp size which when digested with Hbl1 resulted in three fragments of 96, 179 and 332 bp corresponding to two restriction sites at 333 and 512 bp for Aspergillus terreus.
For example, aspA was represented by five alleles and the locus was analyzed in three fragments (Table 1).
To mutate internal Age I, Xho I, and Bgl II sites of the pyrimethamine resistant Toxoplasma gondii dihydrofolate reductase-thymidylate synthase gene (TgDHFR-TS, 1.846 kb), the ORF was amplified in three fragments (TgDTA: F1/R1; TgDTB: F2/R2; F3/R3) using Phusion DNA polymerase and primers containing one base silent mutations corresponding to the restriction enzyme sites.
The complete sequence of C. verrucosa was therefore obtained in three fragments.
The candidate region was subcloned from the BAC clone HWC026-A20 in three fragments, namely Comp.1, Comp.2, and Comp.3.
Similar(48)
One letter was discovered to be in five fragments in five different libraries.
Digestion with Sac I resulted in two fragments of 1.5 and 10 kb hybridizing to the probes.
Sampling was performed during rainy and dry season of 2010 in six fragments of Atlantic forest, using pitfall traps baited with excrement and carrion.
We measured 5297 stems sampled in four categories replicated in eight fragments: fragment interiors (>110 m from edges); old (>50 years) corner edges (< 50 m); old straight edges; and new (ca. 44 years) straight edges.
We inventoried a total of 1057 myrmecophytes in four fragments and four continuous forest sites; the twelve plant species recorded were occupied by 33 ant morphospecies, of which 11 were obligate plant inhabitants.
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