It was completed by a team of 14 annotators in three annotation rounds and provides a high-quality, reliable and consistently annotated resource.
In one annotation, Mr. Carter winces that the diary entries do seem self-congratulatory.
(In one annotation, she suggests a possible explanation for her skill in structuring a story: "Can you tell I watched a lot of soap operas as a kid?") There's a lot to unpack from Colloff's reporting of the wrongful conviction of Michael Morton, who served 25 years in prison for his wife's murder.
To receive a more comprehensive and structured view of the annotation terms a DAVID clustering analysis under high stringency conditions was performed resulting in ten annotation clusters matching the statistical criteria [(p<0.05), fold enrichment >1.5 and an enrichment score of at least 2.0].
However, 89.7 percent of all predicted protein coding genes shared the same stop sites in all three annotations.
Of the genes with identical reading frames in all three annotations, those called by RAST had the longest average length; 967 base pairs for RAST, 940 for JCVI, and 934 for IMG.
Surprisingly, out of the collective 776 suggested reactions, we found only 264 reactions (34%) present in all three annotations.
Specifically, we used the Fisher's exact probability test to determine whether the set of overlapping reactions (those found in all three annotation databases) was "enriched" for active use in a simulation.
This differentiation in gene function among the three clusters was commonly observed in all three annotation types.
We found that under these criteria, a match to the RBS was present upstream of fewer than 10 percent of the predicted genes in each of the three annotations (IMG: 8.6%, RAST: 8.1%, JCVI: 8.6%).
Interestingly, reactions found in all three annotation databases proved to contribute significantly to the actively used reactions in validation simulations.
