Only four pet genes (cytochrome b6f complex) displayed significant variations in their transcript abundance.
These include psbD, psbD2, psbQ, psbW, psb27 and psb29, which displayed significant changes in their transcript patterns with maximum values at 06 30 or 07 30 (Fig. 5).
More than 25% of the genes displayed significant variations in their transcript abundance during the 24 h cycle, most of which occurred following the dark/light transition.
Finally, the two PKS I gene clusters (PKSI modular/PKSIII and PKSI iterative) did not show any significant variation in their transcript levels during the light/dark cycle (Table S2).
This first application of transcriptomics to a Microcystis species has revealed that more than 25% of the genes displayed significant changes in their transcript abundance during the light/dark cycle and in particular during the dark/light transition.
Unfortunately the terminal sampling procedure precludes us from testing this directly but it is likely that dominants who fall in status will also exhibit a profound change in their transcript profile correlated with their lower status.
Among the sig genes identified in the genome of M. aeruginosa PCC 7806, four sigma factors, sigB, sigD, sigE and sigH, displayed significant changes in their transcript levels during the light/dark cycle (Fig. 9B).
Three ABC-type phosphate transporter genes (pst) displayed only slight variations in their transcript abundance, which was characterized by a slight increase between 00 00 and 07 30, followed by a decrease.
More importantly, we conclude from the observation that S11-RNase mRNA levels decrease but S11-RNase levels do not that the reduction in levels of non-self S-RNases are not simply due to a reduction in their transcript levels in the style.
We found also that among these 1344 genes, 40% displayed a zenith (peak-up) or a nadir (peak down) in their transcript abundance at 07 30 (i.e. 1h30 after the transition from dark to light) knowing that most of them were characterized by a maximum transcript abundance (Fig. 2A).
Both genes show strong tissue specific differences in their transcript abundance.
