Exact(13)
The cytoplasmic fluorescence intensity remains constant in the unbleached cells.
Within each cell, we selected five boxed ROIs in the bleached cell region, and five boxed ROIs in the unbleached cell region.
In cells which were in the process of septation, fluorescence recovery (recovery half-time τ1/2: ∼8 s) was observed in the bleached half, and the corresponding fluorescent decay was observed in the unbleached half (Figure 2b, c&d).
First, the tip to rear gradient of EGFP-actin fluorescence in the unbleached regions of the lamellipodium could be preserved, indicating retention of the main component of F-actin (Figure 2).
As the PBsome complexes diffuse upon the membrane surface, one should observe: (1) a fluorescence loss in the unbleached area if the PBsomes diffuse across the cells (Figure 4A C); (2) successive flattening of the slope of the fluorescence intensity gradient (Figure 4A C); (3) changes of the recovery rate as a function of distance from the edge of the bleached area (Figure 4D F).
Comparative analysis of unnormalized intensities in the unbleached tip region and cytoplasm.
Similar(47)
Top panel: initial image acquired from muscle fibre tip 7 showing the area to be bleached in green and the unbleached tip region in red.
When they checked, Vibrio was present in all the summertime samples of the bleached coral, but in none of the unbleached coral.
In many cases, the unbleached ROI shows a small, negative FRET value.
This approach uses the CFP-YFP double tag and the selective bleaching of the YFP moiety in the cytoplasm, while the unbleached CFP serves as an intramolecular control.
This resulted in a reduction in the fluorescence intensity of the unbleached nuclear area as well.
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