The authors determined those patterns for the most frequent TDR mutations against NRTIs (variants/revertants at amino acid 215 in the reverse transcriptase [RT]), nonnucleoside reverse transcriptase inhibitors (NNRTIs) (103N in RT), and protease inhibitors (90M in the protease [PR]).
Three negative controls (with no template, no reverse transcriptase and no RNA in the reverse transcriptase reaction) were also included in each plate to detect any possible contamination.
Moreover, an HIV-1 strain with decreased susceptibility to SPL2923 has been reported that harbors mutations in gp120 but not in the reverse transcriptase indicating lack of selective pressure of the dendrimer against the reverse transcriptase target [11].
Background: Specific mutations in the reverse transcriptase (RT) gene of HIV-1 are associated with reduced activity of nucleoside inhibitors used in the antiretroviral treatment of infected patients.
Study design: The 2001 program included three challenges, each comprised of five blind-labeled panel members containing mutations in the reverse transcriptase (RT) and/or protease (PR) genes.
Sequence comparisons placed the telomerase proteins in the reverse transcriptase family but revealed hallmarks that distinguish them from retroviral and retrotransposon relatives.
We demonstrate that site-directed chemical modification (SDCM) of the RNase H domain by selectively PEGylating C635, one of the eight cysteine residues present in the reverse transcriptase (RT), specifically inactivated its ribonucleolytic activity.
Genotypic data consisted of the binary-coded presence/absence of 54 mutations in the reverse transcriptase (RT) and protease (PR) genes.
HIV resistance to AZT is commonly associated with a cluster of mutations in the reverse transcriptase, known as TAMs (thymidine analog mutations).
They detect mutations in the reverse transcriptase and protease genes.
