In the second assay for alkaloids, cough frequency and latent period of cough were recorded.
In the first assay, a commonly used design was followed, in which the aptamer and target were incubated to allow binding followed by exposure to the AuNPs.
In the first assay, cell subsets were identified using fluorescently labeled antibodies, and viability was determined by staining with 50 μg/mL of propidium iodide (PI) to monitor losses in cell membrane integrity.
In the first assay, 4 duodenally cannulated Holstein steers housed in metabolism cages, dosed with C32 n-alkane, and fed Avena strigosa plus concentrate and increasing levels of tannin extract to reduce ruminal digestibility, were used in a Latin square design.
In the second assay, the ABA inhibition of primary root growth was measured.
Allowing cultivars a one-week head start in the second assay reduced the time Pseudonocardia had to produce antibiotics, resulting in less strong inhibition (Fig. 6B).
In the first assay, the percentage of seedlings with green cotyledons was scored from seeds germinated on MS/G plates containing 1.0 µM ABA for 11 days.
In the second assay, we added 16 1 2-day-old non-virgin LHM-bw females to 1 2-day-oldold LHM males (1 2-day-old generations 4–7).
In the first assay, fixed and permeabilized HSV-2 plaques were incubated with 1∶5,000 dilutions of mouse serum, and stained with Alexa Fluor-594-conjugated secondary antibody.
