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In the testing trial (the test phase), performed 24 hours later, one of the familiar objects was changed for another new, and the animals were left in the OF during 15 min. The exploration time for the familiar (TF) or the new object (TN) during the test phase was recorded.
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In the test trial, we found that the center of mass of the trunk accelerated backward, and induces falling backward.
In the test trial, we found that an additional point is needed to support the body while the spring coefficient is set lower, assuming that the body is supported by a hand.
This possible explanation is supported by the fact that 5 of the 9 wolves responded already at the age of 4 months to the gaze cue of the dog demonstrator, moving around the barrier either only in the test trial or in the test trial earlier than in the control trial.
Thus, latent learning was assessed by recording the number of approaches in the training trial and entering, finding and drinking latencies in the test trial.
In the test trial (day 22), the trained or non-trained mice were placed in the same corner of the test apparatus.
When only the test trial included a CS response ('0-1', yellow trace), simple-spike firing rate was higher in the test trial and the trial-over-trial change in simple-spike firing rate was more positive.
The simple-spike firing rate was higher in test trials that contained a CS response, and the trial-over-trial change in firing rate was more positive, compared to pairs that did not contain a CS response in the test trial.
The end of the water tube is set further above the floor in the test trial than in training to decrease the probability of it being found by chance [ 29].
A drinking tube, identical to that used in the home cage, was inserted into the center of the alcove ceiling with its tip 5 cm (in the training trial) or 7 cm (in the test trial) above the floor.
The new stimuli replaced S+ in half of the testing trials and replaced the S- in the other half.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com